This PCR kit can detect and differentiate the agents in 3 hours as compared to 3-5 days using the conventional methods. The inclusion of internal amplification control can avoid false negative and produce more reliable results. The test is easy to perform in any laboratory equipped with a thermocycler and simple electrophoretic facility. This PCR kit can be used to detect pathogens in clinical, food and environmental samples. This kit is useful for quick and early detection of typhoid/paratyphoid fever and the source of potential outbreak. It can also be used for carrier screening among food handlers. In the absence of serotyping facilities, identification of Salmonella Typhi and Paratyphi A is possible with this PCR kit.
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Lee Ching Shya
UMCIC Business Officer University of Malaya