Burkholderia pseudomallei is a Gram negative motile bacillus and an environmental saprophyte that causes melioidosis. While the disease is commonly reported in Southeast Asia and Northern Australia, an alarming rate of clinical cases are being reported in other regions like America, Africa and India. Current diagnosis tools of melioidosis include culture, biochemical profile, serology test and molecular identification through Polymerase Chain Reaction (PCR). In our study, we have developed an insulated isothermal PCR (iiPCR) assay in identifying Burkholderia pseudomallei, which are accurate, cost effective and user friendly. The iiPCR system is able to complete diagnosis of Burkholderia pseudomallei in an hour.
This end-point detection platform is user friendly. Impacts of the product include quick diagnosis of melioidosis, cost effective, labor saving and time effective. In addition, field scientists are able to make quick countermeasure or preventive decision based on the results obtained on the spot the samples are taken (contaminated soil and water).
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For any inquiries, kindly contact:
Lee Ching Shya
UMCIC Business Officer University of Malaya