Synthetic Biology Biosensor (CYCsensor) for the Discovery of Quorum Sensing Inhibitors

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1. Technology Overview

The present invention generally relates to a synthetic biology biosensor (Cycsensor) for discovery of quorum sensing inhibitors. Accordingly, the synthetic biology biosensor (Cycsensor) for discovery of quorum sensing inhibitors, the Cycsensor includes, a) araC gene (100) encoding for AraC repressor protein for repressing expression of signal synthase gene driven from PβAD promoter, wherein inducible promote  PβAD drives downstream gene expression in presence or arabinose; b) representative rhll from Pseudomonas aeruginosa for C4-HSL production, wherein the repsentative rhll is driven by PβAD promoter in presence of arabinose; c) representative rhlR from Pseudomonas aeruginosa for responding to C4-HSL, whrein the representative rhlR is driven from a constitutive promoter; and d) representative green fluorescent protein gene, gfp, wherein the representative green fluorescent protene gene, gfp is expressed from the responding Prhl promoter which is only functional in presence of RhIR/C4-HSL complex.

  • UMCIC File Number: 603/985
  • Patent: PI 2016 703909
  • Patent status: Pending
  • Malaysia patent: Synthetic Biology Biosensor (CYCsensor) for the Discovery of Quorum Sensing Inhibitors
  • This technology is currently available for licensing and commercialisation.

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