One Step Multiplex System for the Detection of Salmonella Spp., S. Typhi and S. Paratyphi A with an Internal Control


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1. Technology Overview

Enteric fever, more commonly known as typhoid fever, caused by S. Typhi, S. Paratyphi A, B and C, is on the rise especially in countries like Pakistan, India, Vietnam, Nepal and China. The clinical diagnosis of typhoid fever is difficult due to overlapping signs and symptoms with other common febrile illness. In addition, the conventional method of detection which is based on physical characteristics and blood tests is slow, requiring at least two days and lacks sensitivity and specificity. That delay is enough to endanger the lives of those suffering with it. Unlike the conventional method, the present invention works easier as it can be applied directly to bacterial DNA obtained from blood and fecal specimen. Identification test results are obtained within approximately four hours. This invention refers to a method that seeks to detect typhoid fever-causing bacteria through a single step multiplex system that is both fast and accurate.  The specificity of the PCR developed will become a better control and surveillance of S. Typhi and S. Paratyphi A.


2. Technology Features and Specifications
  • Fast
    • The entire test takes approximately four hours as opposed to a minimum of 48 hours when using conventional methods
  • Accurate
    • The test comprises of fragments to test for Salmonella , S. Typhi and S Paratyphi.
    • If the samples show matches for any of the fragments, the test is able to detect it.
    • Minute amounts of the bacteria are detectable.
    • Able to detect the onset of infection
    • Detection is not hindered by medicating the patient


3. Market Opportunity and Potential Applications

Reported cases of enteric fever caused by Samonella spp., S. Typhi and S. Paratyphi A is increasing in recent years. Slow diagnosis has resulted in an increase in mortality. In order to provide quick treatment and appropriate medicine, application of this new technique is necessary. Furthermore, this technique can be applied to detect other diseases by changing the primers into those which fit to detect the particular microorganism which causes the disease.


4. Benefits and Impacts

As this technology can detect the presence of the bacteria in a very short time, precise diagnostic can be done at the early stage. Hence, appropriate and prompt treatment with antimicrobial agents can be given to the patients immediately after diagnosis. This can effectively reduce the mortality rate caused by the enteric fever.


5. Development Stage

Available for Licensing

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